Nwuike, who drove a luxury bus with registration no. LND 922 XN recklessly and hit Gladys while she was crossing the highway.
The accused, who works for the Young Motors, is facing a two-count charge of dangerous driving and manslaughter.
He, however, pleaded not guilty to the charges.
But the prosecutor, Sgt. Kehinde Olatunde, told the court that the accused committed the offences on June 30 about 7.20 p.m. along Badagry Expressway by Suru-Alaba Bus Stop.
He said Nwuike, who drove a luxury bus with registration no. LND 922 XN recklessly and hit Gladys.
Olatunde said accused hit the deceased while she was crossing the highway.
The offences contravened Sections 19 and 20 of the Road Traffic Laws of Lagos State, 2012.
In her ruling, the Magistrate, Mrs Bola Folarin-Williams admitted the accused to a bail of N500, 000 with two responsible sureties in like sum.
She directed the court officials to duplicate the case file and send a copy to the State Director of Public Prosecutions for advice.
The case was adjourned until September 20.