Kwadwo Asamoah Asamoah was an unused substitute as Paulo Dybala inspired Juventus to a 3-0 win to leave Luis Enrique in need of another miracle
Luis Enrique's side made history by overturning a 4-0 deficit to knock PSG out 6-1 in the last round of the competition, but they will have to perform a similar feat at the Nou Camp next Wednesday after a dismal night in Turin.
Dybala got the ball rolling with an impressive first-half brace, and Giorgio Chiellini added a third with a header after the break for Juventus in the first meeting between the sides since Barcelona's win in the 2015 Champions League final.
It is scarcely credible to imagine Massimilino Allegri's streetwise Juventus defence, marshalled superbly by Leonardo Bonucci and Giorgio Chiellini – who scored the third 10 minutes after half-time – folding as the Ligue 1 champions did in the Camp Nou cauldron.
Outgoing Barca boss Luis Enrique once again deployed the hybrid 3-4-3 he fielded to see off PSG but the result was a disorganised rabble that was picked apart by Serie A's dominant force.
The visitors were relieved to see Gonzalo Higuain plant his second-minute header from Miralem Pjanic's s free-kick straight at Marc-Andre ter Stegen, although another Argentinian proved more clinical five minutes later.
Jeremy Mathieu – a surprise selection on the left-hand side of defence – allowed Juan Cuadrado into the box and Dybala collected the pass to turn and finish on the angle with almost nonchalant cool.