The Italian champions were already through to the last 16, along with Group G leaders Barcelona, but can still pip the Spaniards to top spot, sitting three points behind them ahead of their trip to the Camp Nou next Tuesday.
"Another barrier has been broken down, we know that she is very good, which is the most important and fundamental thing," Juventus chief football officer Fabio Paratici said before the game of Frappart.
The 36-year-old Frappart has already made history as the first woman to referee in Ligue 1, and took charge of the 2019 UEFA Super Cup final between Liverpool and Chelsea. She also made her Europa League debut in October.
Juventus had few problems on the pitch against the Ukrainians who they had also beaten 2-0 in Kiev on October 20.
Federico Chiesa inspired the victory, scoring the opening goal on 21 minutes helping Ronaldo and Alvaro Morata add two more in the second half.
Dynamo goalkeeper Georgiy Bushchan kept out a Weston McKennie header on 17 minutes.
But five minutes later, ex-Fiorentina midfielder Chiesa got his head to an Alex Sandro cross to beat Bushchan for his first goal since joining Juventus two months ago.
Ronaldo hit the woodwork after half an hour, with Buschan also denying the Portuguese star early in the second half.
The visitors had few chances but Wojciech Szczesny did well to prevent Viktor Tsygankov from scoring an equaliser before the break.
Just before the hour mark, Italy's Chiesa powered down the right wing before sending in a low cross for Morata which Bushchan pushed into the path of Ronaldo to tap in from close range.
The 35-year-old extended his Champions League record to 132 goals in the competition, and brought his tally for club and country to 750 goals.
Chiesa was again involved in the third goal with 25 minutes remaining, picking out the run of Morata, who took three touches before firing in his sixth Champions League goal this season, having also scored a brace in Ukraine.