The two-time defending Games 100-metre champion withdrew with an "unspecified injury", according to the team.
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Injured Usain Bolt out of Jamaica trials ahead of Games
Usain Bolt is battling injury ahead of the Olympics after withdrawing from the Jamaica team trials on Friday.
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Bolt is widely expected to make it three straight gold medals in the event in Rio de Janeiro, but his latest injury is a blow to his chances.
The 29-year-old had won his semi-final at the trials in a time of 10.04 seconds.
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