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According to the Aden governor's office and medics, no fewer than 71 people were said to have been killed in the attack, and at least 33 others were wounded.
Report said the car bomb exploded inside a training camp for forces allied to Saudi-backed Yemeni President Abdu Rabu Mansour Hadi.
The explosion reportedly occurred around 8:15 a.m. local time while recruits were waiting in line to be enrolled among troops heading to battle at the Saudi-Yemeni border, officials said.
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Aden has been the de-facto capital of Hadi's Saudi-backed government. The actual capital, Sanaa, has been under the control of Houthi rebels since last year.
Monday's attack is the latest in the country's civil war.
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