Mario Mandzukic scored in the 59 minute to inspire Juventus to a 1-0 win against the Spaniards.
Cristiano Ronaldo sets new Champions League record after Juventus win against Valencia
The Portuguese skipper is the first player to record 100 UEFA Champions League wins after Juventus pipped Valencia 1-0.
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The former Real Madrid star is also the competition's all-time top-scorer, having netted 121 goals so far and the player with the second highest appearances in the competition, only behind legendary goalkeeper Iker Casillas.
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Within his distinguished career, Ronaldo picked up 26 wins with Manchester United, 71 with Real Madrid and three with Juve, which has moved them to the knock-out stage of the competitions.
"Ronaldo gives us a sense of security and confidence," he said after the game, as quoted by Football Italia.
"The team is essentially the same as last season. We've grown, become faster in playing the ball, but then of course, there is Cristiano Ronaldo.
"This team is constantly improving – they're passing and putting play together more quickly."
Ronaldo is now targeting a sixth Champions League title to add to his four with Los Blancos and one with United.
While the Bianconeri are aiming for their third European title and first since 1995.
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