They were trapped and killed after the fire gutted their house at Kokomlemle at about 10:30 pm on Friday.
Ten-years Junior Okai and his brother Stephen Okai, were said to be asleep in their room when the incident occurred, according to Joy News.
The children were heard calling for help according to eyewitnesses.
Meanwhile, three other family members have sustained various degrees of injury, an official of the Ghana National Fire Service, Eugene Ahomi, has said.
Ahomi also told Joy News the cause of the fire is yet to be established.