Andre Gomes, Denis Suarez and Jeremy Mathieu were among those featuring, but Felipe Caicedo's early goal was decisive in Tarragona.
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Barcelona downed by Espanyol in Supercopa Catalunya
Barcelona suffered a 1-0 loss to Espanyol in the Supercopa Catalunya final as Luis Enrique made plenty of changes on Tuesday.
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A Jose Antonio Reyes pass found Caicedo, who rounded goalkeeper Jordi Masip for the game's only goal in the 10th minute.
Barcelona return to action on Saturday when they host Granada in LaLiga.
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